| 51 | Olga Zaytseva |  | 49.49 | | Jul 16, 2006 | Tula (RUS) |
| 51 | Henriette Jæger |  | 49.49 | | Aug 28, 2025 | Letzigrund, Zürich (SUI) |
| 53 | Candice Mcleod |  | 49.51 | | Aug 4, 2021 | National Stadium, Tokyo (JPN) |
| 54 | Shakima Wimbley |  | 49.52 | | Jun 23, 2018 | Drake Stadium, Des Moines, IA (USA) |
| 55 | Beatrice Masilingi |  | 49.53 | | Apr 11, 2021 | National Heroes Stadium, Lusaka (ZAM) |
| 55 | Vanya Stambolova |  | 49.53 | | Aug 27, 2006 | Rieti (ITA) |
| 57 | Monique Hennagan | | 49.56 | | Jul 17, 2004 | Sacramento, CA (USA) |
| 57 | Bärbel Wöckel |  | 49.56 | | May 30, 1982 | Erfurt (GER) |
| 59 | Grace Jackson | | 49.57 | | Jul 10, 1988 | Nice (FRA) |
| 59 | Athing Mu | | 49.57 | | Jun 12, 2021 | Hayward Field, Eugene, OR (USA) |
| 61 | Lieke Klaver |  | 49.58 | | Jul 20, 2024 | Olympic Stadium, London (GBR) |
| 61 | Sada Williams |  | 49.58 | | Aug 21, 2023 | Nemzeti Atlétikai Központ, Budapest (HUN) |
| 61 | Dagmar Neubauer | | 49.58 | | Jun 2, 1984 | Erfurt (GER) |
| 64 | Stacey Ann Williams | | 49.59 | | Sep 14, 2025 | National Stadium, Tokyo (JPN) |
| 64 | Katharine Merry |  | 49.59 | | Jun 11, 2001 | Athina (GRE) |
| 64 | Marion Jones | | 49.59 | | Apr 16, 2000 | Walnut, CA (USA) |
| 67 | Wadeline Jonathas | | 49.60 | | Oct 3, 2019 | Khalifa International Stadium, Doha (QAT) |
| 68 | Phyllis Francis | | 49.61 | | Oct 3, 2019 | Khalifa International Stadium, Doha (QAT) |
| 68 | Ana Fidelia Quirot |  | 49.61 | | Aug 5, 1991 | La Habana (CUB) |
| 70 | Caster Semenya |  | 49.62 | | Sep 8, 2018 | Ostrava (CZE) |
| 71 | Novlene Williams-Mills | | 49.63 | | Sep 23, 2006 | Shanghai (CHN) |
| 72 | Gwen Torrence | | 49.64 | | Jul 15, 1992 | Nice (FRA) |
| 72 | Debbie Dunn | | 49.64 | | Jun 26, 2010 | Drake Stadium, Des Moines, IA (USA) |
| 72 | DeeDee Trotter | | 49.64 | | Jun 23, 2007 | Indianapolis, IN (USA) |
| 72 | Ximena Restrepo |  | 49.64 | | Aug 5, 1992 | Estadio Olímpico, Barcelona (ESP) |
| 76 | Dejanea Oakley | | 49.65 | | Jun 14, 2025 | Hayward Field, Eugene, OR (USA) |
| 76 | Nicola Sanders | | 49.65 | | Aug 29, 2007 | Yanmar Stadium Nagai, Osaka (JPN) |
| 76 | Natalya Nazarova | | 49.65 | | Jul 31, 2004 | Tula (RUS) |
| 79 | Christina Brehmer | | 49.66 | | Jul 28, 1980 | Lenin Stadium, Moskva (URS) |
| 79 | Lillie Leatherwood | | 49.66 | | Jun 15, 1991 | New York, NY (USA) |
| 81 | Sandra Myers |  | 49.67 | | Jul 6, 1991 | Oslo (NOR) |
| 82 | Shamier Little | | 49.68 | | Jul 21, 2023 | Stade Louis II, Monaco (MON) |
| 83 | Courtney Okolo | | 49.71 | | Apr 23, 2016 | Baton Rouge, LA (USA) |
| 84 | Rosey Effiong | | 49.72 | | Jun 8, 2024 | Hayward Field, Eugene, OR (USA) |
| 84 | Quanera Hayes | | 49.72 | | Jun 24, 2017 | Sacramento, CA (USA) |
| 84 | Martina Weil |  | 49.72 | | Aug 28, 2025 | Letzigrund, Zürich (SUI) |
| 87 | Anja Rücker | | 49.74 | | Aug 26, 1999 | La Cartuja, Sevilla (ESP) |
| 88 | Gaby Bussmann | | 49.75 | | Aug 10, 1983 | Olympiastadion, Helsinki (FIN) |
| 89 | Alexis Holmes | | 49.77 | | Aug 9, 2024 | Stade de France, Paris (FRA) |
| 89 | Olga Kotlyarova | | 49.77 | | Jul 2, 2004 | Stadio Olimpico, Roma (ITA) |
| 91 | Sandie Richards | | 49.79 | | Aug 4, 1997 | Olympic Stadium, Athina (GRE) |
| 91 | Donna Fraser | | 49.79 | | Sep 25, 2000 | Olympic Stadium, Sydney (AUS) |
| 93 | Evelis Aguilar | | 49.80 | | Apr 6, 2025 | Estadio de Atletismo - Unidad Deportiva el Salitre, Bogotá (COL) |
| 93 | Fiordaliza Cofil |  | 49.80 | | Sep 2, 2022 | Boudewijnstadion, Bruxelles (BEL) |
| 93 | Lynna Irby | | 49.80 | | Jun 9, 2018 | Eugene, OR (USA) |
| 93 | Kseniya Ryzhova | | 49.80 | | Jun 24, 2013 | Cheboksary (RUS) |
| 93 | Svetlana Pospelova | | 49.80 | | Jul 11, 2005 | Tula (RUS) |
| 98 | Yuqin Ma |  | 49.81 | | Sep 11, 1993 | Beijing (CHN) |
| 99 | Natasha Hastings | | 49.84 | | Jun 23, 2007 | Indianapolis, IN (USA) |
| 99 | Rose-Mary Whyte | | 49.84 | | Aug 6, 2011 | Crystal Palace, London (GBR) |
