| 04 | Jarmila Kratochvílová |  | 47.99 | | 10 ago 1983 | Olympiastadion, Helsinki (FIN) |
| 05 | Salwa Eid Naser |  | 48.14 | | 3 oct 2019 | Khalifa International Stadium, Doha (QAT) |
| 06 | Marie-José Pérec |  | 48.25 | | 29 jul 1996 | Olympic Stadium, Atlanta, GA (USA) |
| 07 | Olga Bryzgina |  | 48.27 | | 6 oct 1985 | Bruce Stadium, Canberra (AUS) |
| 08 | Shaunae Miller-Uibo |  | 48.36 | | 6 ago 2021 | National Stadium, Tokyo (JPN) |
| 09 | Nickisha Pryce |  | 48.57 | | 20 jul 2024 | Olympic Stadium, London (GBR) |
| 10 | Taťána Kocembová | | 48.59 | | 10 ago 1983 | Olympiastadion, Helsinki (FIN) |
| 11 | Cathy Freeman |  | 48.63 | | 29 jul 1996 | Olympic Stadium, Atlanta, GA (USA) |
| 12 | Sanya Richards-Ross |  | 48.70 | | 16 sept 2006 | Olympic Stadium, Athina (GRE) |
| 13 | Valerie Brisco-Hooks | | 48.83 | | 6 ago 1984 | Memorial Coliseum, Los Angeles, CA (USA) |
| 14 | Ana Gabriela Guevara |  | 48.89 | | 27 ago 2003 | Stade de France, Paris-St-Denis (FRA) |
| 15 | Natalia Kaczmarek |  | 48.90 | | 20 jul 2024 | Olympic Stadium, London (GBR) |
| 16 | Chandra Cheeseborough | | 49.05 | | 6 ago 1984 | Memorial Coliseum, Los Angeles, CA (USA) |
| 17 | Tonique Williams-Darling | | 49.07 | | 12 sept 2004 | Olympiastadion, Berlin (GER) |
| 17 | Rhasidat Adeleke |  | 49.07 | | 10 jun 2024 | Stadio Olimpico, Roma (ITA) |
| 19 | Aaliyah Butler | | 49.09 | | 11 jul 2025 | Stade Louis II, Monaco (MON) |
| 20 | Falilat Ogunkoya-Osheku |  | 49.10 | | 29 jul 1996 | Olympic Stadium, Atlanta, GA (USA) |
| 21 | Olga Nazarova | | 49.11 | | - | - |
| 22 | Kaylyn Brown | | 49.13 | | 8 jun 2024 | Hayward Field, Eugene, OR (USA) |
| 22 | Britton Wilson | | 49.13 | | 13 may 2023 | LSU Bernie Moore Stadium, Baton Rouge, LA (USA) |
| 24 | Gabrielle Thomas | | 49.14 | | 5 abr 2025 | National Stadium, Kingston (JAM) |
| 25 | Antonina Krivoshapka | | 49.16 | | 5 jul 2012 | Cheboksary (RUS) |
| 26 | Femke Bol |  | 49.17 | | 2 mar 2024 | Glasgow Arena, Glasgow (GBR) (i) |
| 27 | Mariya Pinigina | | 49.19 | | 10 ago 1983 | Olympiastadion, Helsinki (FIN) |
| 28 | Christine Mboma |  | 49.22 | | 17 abr 2021 | Independence Stadium, Windhoek (NAM) |
| 29 | Isabella Whittaker | | 49.24 | | 15 mar 2025 | Virginia Beach Sports Center, Virginia Beach, VA (USA) (i) |
| 29 | Sabine Busch |  | 49.24 | | 2 jun 1984 | Erfurt (GER) |
| 31 | Allyson Felix | | 49.26 | | 27 ago 2015 | National Stadium, Beijing (CHN) |
| 32 | Pauline Davis-Thompson | | 49.28 | | 29 jul 1996 | Olympic Stadium, Atlanta, GA (USA) |
| 33 | Irena Szewińska | | 49.29 | | 29 jul 1976 | Olympic Stadium, Montréal (CAN) |
| 33 | Charity Opara | | 49.29 | | 14 jul 1998 | Stadio Olimpico, Roma (ITA) |
| 33 | Amber Anning |  | 49.29 | | 9 ago 2024 | Stade de France, Paris (FRA) |
| 36 | Petra Schersing | | 49.30 | | 3 jun 1988 | Jena (GDR) |
| 36 | Lorraine Fenton | | 49.30 | | 19 jul 2002 | Stade Louis II, Monaco (MON) |
| 38 | Shericka Williams | | 49.32 | | 18 ago 2009 | Olympiastadion, Berlin (GER) |
| 39 | Amantle Montsho |  | 49.33 | | 19 jul 2013 | Stade Louis II, Monaco (MON) |
| 40 | Stephenie Ann Mcpherson | | 49.34 | | 4 ago 2021 | National Stadium, Tokyo (JPN) |
| 41 | Jearl Miles-Clark | | 49.40 | | 14 jun 1997 | Indianapolis, IN (USA) |
| 42 | Christine Ohuruogu | | 49.41 | | 12 ago 2013 | Luzhniki Stadion, Moskva (RUS) |
| 43 | Grit Breuer | | 49.42 | | 27 ago 1991 | National Stadium, Tokyo (JPN) |
| 44 | Fatimat Yusuf | | 49.43 | | 15 sept 1995 | Harare (ZIM) |
| 44 | Kathy Cook | | 49.43 | | 6 ago 1984 | Memorial Coliseum, Los Angeles, CA (USA) |
| 46 | Kendall Ellis | | 49.46 | | 23 jun 2024 | Hayward Field, Eugene, OR (USA) |
| 47 | Shericka Jackson | | 49.47 | | 3 oct 2019 | Khalifa International Stadium, Doha (QAT) |
| 47 | Aelita Yurchenko | | 49.47 | | 4 sept 1988 | Moskva (URS) |
| 49 | Roxana Gómez |  | 49.48 | | 18 sept 2025 | National Stadium, Tokyo (JPN) |
| 49 | Francena Mccorory | | 49.48 | | 28 jun 2014 | Sacramento, CA (USA) |
